Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Association between acetaminophen metabolites and CYP2E1 DNA methylation level in neonate cord blood in the Boston Birth Cohort Clinical Epigenetics Springer Nature Link Glutathione, Alcohol and drugs Tylenol Glutathione Kizer Wellness LLC Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research